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Human FLRT3 ELISA Kit PicoKine®, Carrier-free

Selected Variant

EK1974-carrier-free

Size: 96 Well With Removable Strips

Human FLRT3 ELISA Kit PicoKine® (96 Tests). Quantitate Human FLRT3 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Leucine-rich repeat transmembrane protein FLRT3 is a protein that in humans is encoded by the FLRT3 gene. It is mapped to 20p12.1. This gene encodes a member of the fibronectin leucine rich transmembrane protein (FLRT) family. FLRTs may function in cell adhesion and/or receptor signalling. Their protein structures resemble small leucine-rich proteoglycans found in the extracellular matrix. This gene is expressed in many tissues. Two alternatively spliced transcript variants encoding the same protein have been described for this gene.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>206</td><td>228</td><td>212</td><td>203</td><td>212</td><td>9.65</td><td>4.5%</td></tr><tr><td>Sample 2</td><td>1152</td><td>1104</td><td>1147</td><td>1147</td><td>1137</td><td>19.44</td><td>1.7%</td></tr><tr><td>Sample 3</td><td>4318</td><td>3995</td><td>4413</td><td>3635</td><td>4090</td><td>305.10</td><td>7.4%</td></tr></table>*number of samples for each test n=16.

Synonyms

Leucine-rich repeat transmembrane protein FLRT3; Fibronectin-like domain-containing leucine-rich transmembrane protein 3; FLRT3; KIAA1469; UNQ856/PRO1865

Gene Name

fibronectin leucine rich transmembrane protein 3

Gene ID

23767

UniProt

Q9NZU0

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: K29-S649

Tissue Specificity

Expressed in kidney, brain, pancreas, skeletal muscle, lung, liver, placenta, and heart.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA).

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<50 pg/ml

Function

Functions in cell-cell adhesion, cell migration and axon guidance, exerting an attractive or repulsive role depending on its interaction partners. Plays a role in the spatial organization of brain neurons. Plays a role in vascular development in the retin

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human FLRT3

Anticoagulant

heparin or EDTA

Subcellular Location

Secreted. Endoplasmic reticulum membrane. Cell membrane. Single-pass membrane protein. Focal adhesion. Axon.

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