Human PROC/Protein C ELISA Kit PicoKine®, Carrier-free

Human PROC/Protein C ELISA Kit PicoKine® (96 Tests). Quantitate Human Proc in cell culture supernatants, serum and plasma (heparin, EDTA) and urine. Sensitivity: 0.1ng/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Protein C (PROC), also called PC, is a zymogenic (inactive) protein, the activated form of which plays an important role in regulating blood clotting, inflammation, cell death and maintaining the permeability of blood vessel walls in humans and other animals. The PROC gene is mapped to 2q14.3. This gene encodes a vitamin K-dependent plasma glycoprotein. The encoded protein is cleaved to its activated form by the thrombinthrombomodulin complex. This activated form contains a serine protease domain and functions in degradation of the activated forms of coagulation factors V and VIII. Mutations in this gene have been associated with thrombophilia due to protein C deficiency, neonatal purpura fulminans, and recurrent venous thrombosis.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>1223</td><td>1177</td><td>1347</td><td>1353</td><td>1275</td><td>76.77</td><td>6.0%</td></tr><tr><td>Sample 2</td><td>5529</td><td>5748</td><td>6539</td><td>6544</td><td>6090</td><td>458.09</td><td>7.5%</td></tr><tr><td>Sample 3</td><td>25231</td><td>23258</td><td>22794</td><td>25529</td><td>24203</td><td>1193.03</td><td>4.9%</td></tr></table>*number of samples for each test n=16.

Synonyms

Vitamin K-dependent protein C; Anticoagulant protein C; Autoprothrombin IIA; Blood coagulation factor XIV; Proc

Gene Name

protein C, inactivator of coagulation factors Va and VIIIa

Gene ID

5624

UniProt

P33587

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A43-P461

Tissue Specificity

Plasma; synthesized in the liver.

Applications

ELISA

Standard

50 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA) and urine.

Detection Range

0.78 ng/ml - 50 ng/ml

Sensitivity

<0.1 ng/ml

Function

Protein C is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids. Exerts a protective effect on the endothelial cell barrier function.

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)