Human Serpin D1 ELISA Kit PicoKine®, Carrier-free

Human Serpin D1 ELISA Kit PicoKine® (96 Tests). Quantitate Human SERPIND1 in cell culture supernatants, serum and plasma (EDTA, citrate) and urine. Sensitivity: 0.1ng/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Heparin cofactor II (HCII), a protein encoded by the SERPIND1 gene, is a coagulation factor that inhibits IIa, and is a cofactor for heparin and dermatan sulfate. It is mapped to 22q11.21. This gene belongs to the serpin gene superfamily. Serpins play roles in many processes including inflammation, blood clotting, and cancer metastasis. Members of this family have highly conserved secondary structures with a reactive center loop that interacts with the protease active site to inhibit protease activity. This gene encodes a plasma serine protease that functions as a thrombin and chymotrypsin inhibitor. The protein is activated by heparin, dermatan sulfate, and glycosaminoglycans. Allelic variations in this gene are associated with heparin cofactor II deficiency.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>976</td><td>1160</td><td>971</td><td>1107</td><td>1053</td><td>82.18</td><td>7.8%</td></tr><tr><td>Sample 2</td><td>6436</td><td>5740</td><td>5874</td><td>6193</td><td>6060</td><td>272.05</td><td>4.4%</td></tr><tr><td>Sample 3</td><td>22337</td><td>22710</td><td>20531</td><td>21109</td><td>21671</td><td>885.79</td><td>4.0%</td></tr></table>*number of samples for each test n=16.

Synonyms

Heparin cofactor 2; Heparin cofactor II; HC-II; Protease inhibitor leuserpin-2; HLS2; Serpin D1; HCF2

Gene Name

serpin family D member 1

Gene ID

3053

UniProt

P05546

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: G20-S499

Tissue Specificity

Expressed predominantly in liver. Also present in plasma.

Applications

ELISA

Standard

50 ng/tube

Sample Type

cell culture supernatants, serum and plasma (EDTA, citrate) and urine.

Detection Range

0.78 ng/ml - 50 ng/ml

Sensitivity

<0.1 ng/ml

Function

Thrombin inhibitor activated by the glycosaminoglycans, heparin or dermatan sulfate. In the presence of the latter, HC-II becomes the predominant thrombin inhibitor in place of antithrombin III (AT-III). Also inhibits chymotrypsin, but in a glycosaminogly

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)