Human Cystatin M/Cystatin E/CST6 ELISA Kit PicoKine®, Carrier-free

Human Cystatin M/CST6 ELISA Kit PicoKine® (96 Tests). Quantitate Human CST6 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Cystatin-M is a protein that in humans is encoded by the CST6 gene. It is mapped to 11q13.1. The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. This gene encodes a cystatin from the type 2 family, which is down-regulated in metastatic breast tumor cells as compared to primary tumor cells. Loss of expression is likely associated with the progression of a primary tumor to a metastatic phenotype.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>79</td><td>79</td><td>75</td><td>76</td><td>77</td><td>1.78</td><td>2.3%</td></tr><tr><td>Sample 2</td><td>382</td><td>396</td><td>405</td><td>372</td><td>388</td><td>12.67</td><td>3.2%</td></tr><tr><td>Sample 3</td><td>1415</td><td>1318</td><td>1387</td><td>1330</td><td>1362</td><td>39.97</td><td>2.9%</td></tr></table>*number of samples for each test n=16.

Synonyms

Cystatin-M; Cystatin-6; Cystatin-E; CST6

Gene Name

cystatin E/M

Gene ID

1474

UniProt

Q15828

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: R29-M149

Tissue Specificity

Restricted to the stratum granulosum of normal skin, the stratum granulosum/spinosum of psoriatic skin, and the secretory coils of eccrine sweat glands. Low expression levels are found in the nasal cavity.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA).

Detection Range

46.9 pg/ml - 3,000 pg/ml

Sensitivity

<10 pg/ml

Function

Shows moderate inhibition of cathepsin B but is not active against cathepsin C.

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)