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Human URB/CCDC80 ELISA Kit PicoKine®, Carrier-free

Human URB/CCDC80 ELISA Kit PicoKine® (96 Tests). Quantitate Human CCDC80 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Coiled-coil domain-containing protein 80 is a protein that in humans is encoded by the CCDC80 gene. It is mapped to 3q13.2. Bioinformatics analysis suggests the CCDC80 protein is a peroxiredoxin.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr> <tr><td>Sample 1</td><td>79</td><td>76</td><td>86</td><td>72</td><td>78</td><td>5.11</td><td>6.5%</td></tr> <tr><td>Sample2</td><td>359 </td><td>370</td><td>404</td><td>430</td><td>390</td><td>28.08</td><td>7.2%</td></tr> <tr><td>Sample3</td><td>1459</td><td>1416</td><td>1530</td><td>1436</td><td>1460</td><td>43.04</td><td>2.9%</td></tr></table>*number of samples for each test n=16.

Synonyms

Coiled-coil domain-containing protein 80; Down-regulated by oncogenes protein 1; Up-regulated in BRS-3 deficient mouse homolog; CCDC80; DRO1; URB; HBE245

Gene Name

coiled-coil domain containing 80

Gene ID

151887

UniProt

Q76M96

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: E22-Y950

Tissue Specificity

Expressed in dermal papilla and dermal fibroblasts (at protein level). Expressed in heart, thymus, placenta, pancreas, colon, epithelium, spleen and osteoblasts.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA).

Detection Range

46.9 pg/ml - 3,000 pg/ml

Sensitivity

<10 pg/ml

Function

Promotes cell adhesion and matrix assembly.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human URB/CCDC80

Anticoagulant

heparin or EDTA

Subcellular Location

Extracellular matrix.

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