Human CA3 ELISA Kit PicoKine®, Carrier-free

Human CA3 ELISA Kit PicoKine® (96 Tests). Quantitate Human CA3 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) and urine. Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Carbonic anhydrase III (CA3) is an enzyme that in humans is encoded by the CA3 gene. CA3 is a member of a multigene family that encode carbonic anhydrase isozymes. It is mapped to 8q21.2. The gene spans 10.3 kb and contains seven exons and six introns. Using a cDNA clone of the CA3 gene in the study of human-rodent hybrids, the gene was mapped to chromosome 8 which carries a cluster of CA genes. The expression of the CA3 gene is strictly tissue specific and present at high levels in skeletal muscle and much lower levels in cardiac and smooth muscle. A proportion of carriers of Duchenne muscle dystrophy have a higher CA3 level than normal.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>1301</td><td>1297</td><td>1136</td><td>1156</td><td>1222</td><td>76.83</td><td>6.2%</td></tr><tr><td>Sample 2</td><td>6028</td><td>5779</td><td>6552</td><td>5475</td><td>5958</td><td>394.67</td><td>6.6%</td></tr><tr><td>Sample 3</td><td>24574</td><td>24410</td><td>24517</td><td>21425</td><td>23731</td><td>1332.95</td><td>5.6%</td></tr></table>*number of samples for each test n=16.

Synonyms

Carbonic anhydrase 3; Carbonate dehydratase III; Carbonic anhydrase III; CA-III; CA3

Gene Name

carbonic anhydrase 3

Gene ID

761

UniProt

P07451

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A2-K260

Tissue Specificity

Muscle specific.

Applications

ELISA

Standard

50 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA, citrate) and urine.

Detection Range

0.78 ng/ml - 50 ng/ml

Sensitivity

<50 pg/ml

Function

Reversible hydration of carbon dioxide.

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)