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Human CNDP1/Carnosine Dipeptidase 1 ELISA Kit PicoKine®, Carrier-free

Human CNDP1 ELISA Kit PicoKine® (96 Tests). Quantitate Human CNDP1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Beta-Ala-His dipeptidase is an enzyme that in humans is encoded by the CNDP1 gene. It is mapped to 18q22.3. This gene encodes a member of the M20 metalloprotease family. The encoded protein is specifically expressed in the brain, is a homodimeric dipeptidase which was identified as human carnosinase. This gene contains trinucleotide (CTG) repeat length polymorphism in the coding region.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>5364</td><td>5541</td><td>5698</td><td>5319</td><td>5481</td><td>279.51</td><td>5.1%</td></tr><tr><td>Sample 2</td><td>12789</td><td>12898</td><td>14236</td><td>13621</td><td>13386</td><td>736.23</td><td>5.5%</td></tr><tr><td>Sample 3</td><td>25697</td><td>26546</td><td>26973</td><td>26459</td><td>26419</td><td>1241.68</td><td>4.7%</td></tr></table>*number of samples for each test n=16.

Synonyms

Beta-Ala-His dipeptidase; CNDP dipeptidase 1; Carnosine dipeptidase 1; Glutamate carboxypeptidase-like protein 2; Serum carnosinase; CNDP1; CN1; CPGL2; UNQ1915/PRO4380

Gene Name

carnosine dipeptidase 1

Gene ID

84735

UniProt

Q96KN2

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: S27-H507

Tissue Specificity

Found in serum and adult nervous central system. Absent in serum from patients with homocarnosinosis.

Applications

ELISA

Standard

200 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA).

Detection Range

6.25 ng/ml - 400 ng/ml

Sensitivity

<50 pg/ml

Function

Preferential hydrolysis of the beta-Ala-|-His dipeptide (carnosine), and also anserine, Xaa-|-His dipeptides and other dipeptides including homocarnosine.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human CNDP1

Anticoagulant

heparin or EDTA

Subcellular Location

Secreted

Frequently Asked Questions

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