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Human C1S ELISA Kit PicoKine®, Carrier-free

Selected Variant

EK1934-carrier-free

Size: 96 Well With Removable Strips

Human C1S ELISA Kit PicoKine® (96 Tests). Quantitate Human C1S in cell culture supernatants, serum, plasma (heparin, EDTA or citrate) , urine, human milk. Sensitivity: 0.1ng/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Complement component 1s is a protein involved in the complement system. It is mapped to 12p13.31. This gene encodes a serine protease, which is a major constituent of the human complement subcomponent C1. C1s associates with two other complement components C1r and C1q in order to yield the first component of the serum complement system. Defects in this gene are the cause of selective C1s deficiency.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>1236</td><td>1246</td><td>1393</td><td>1346</td><td>1305</td><td>66.45</td><td>5.0%</td></tr><tr><td>Sample 2</td><td>4483</td><td>4300</td><td>3867</td><td>4110</td><td>4190</td><td>228.40</td><td>5.4%</td></tr><tr><td>Sample 3</td><td>22854</td><td>23761</td><td>20481</td><td>22796</td><td>22473</td><td>1212.07</td><td>5.3%</td></tr></table>*number of samples for each test n=16.

Synonyms

Complement C1s subcomponent; C1 esterase; Complement component 1 subcomponent s; C1S

Gene Name

complement C1s

Gene ID

716

UniProt

P09871

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: E16-D688

Applications

ELISA

Standard

50 ng/tube

Sample Type

cell culture supernatants, serum, plasma (heparin, EDTA or citrate) , urine, human milk

Detection Range

0.78 ng/ml - 50 ng/ml

Sensitivity

<0.1 ng/ml

Function

C1s B chain is a serine protease that combines with C1q and C1r to form C1, the first component of the classical pathway of the complement system. C1r activates C1s so that it can, in turn, activate C2 and C4.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human C1S

Anticoagulant

heparin, EDTA or citrate

Subcellular Location

Blood microparticle, Extracellular region

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