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Mouse REG3A ELISA Kit PicoKine®, Carrier-free

Selected Variant

EK1835-carrier-free

Size: 96 Well With Removable Strips

Mouse REG3A ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Reg3a in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Regenerating islet-derived protein 3 alpha is a protein that in humans is encoded by the REG3A gene. It is mapped to 6 C3; 6 34.76 cM. This gene encodes a pancreatic secretory protein that may be involved in cell proliferation or differentiation. It has similarity to the C-type lectin superfamily. The enhanced expression of this gene is observed during pancreatic inflammation and liver carcinogenesis. Multiple alternatively spliced transcript variants encoding the same protein have been described for this gene but the full length nature of some transcripts is not yet known.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>30</td><td>35</td><td>32</td><td>36</td><td>33</td><td>2.38</td><td>7.2%</td></tr><tr><td>Sample 2</td><td>174</td><td>156</td><td>153</td><td>148</td><td>157</td><td>9.80</td><td>6.2%</td></tr><tr><td>Sample 3</td><td>502</td><td>518</td><td>455</td><td>436</td><td>477</td><td>33.42</td><td>7.0%</td></tr></table>*number of samples for each test n=16.

Synonyms

Regenerating islet-derived protein 3-alpha; REG-3-alpha; Islet of Langerhans regenerating protein 3; Lithostathine 3; Pancreatitis-associated protein 2; Regenerating islet-derived protein III-alpha; Reg III-alpha; Reg3a; Pap2

Gene Name

regenerating islet-derived 3 alpha

Gene ID

19694

UniProt

O09037

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: E27-Q175

Tissue Specificity

Small intestine and pancreas.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin).

Detection Range

15.6 pg/ml - 1,000 pg/ml

Sensitivity

<10 pg/ml

Function

Bactericidal C-type lectin. Regulates keratinocyte proliferation and differentiation after skin injury via activation of EXTL3-PI3K-AKT signaling pathway.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant mouse REG3A

Anticoagulant

heparin

Subcellular Location

Secreted

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