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Human P4HB ELISA Kit PicoKine®, Carrier-free

Selected Variant

EK1890-carrier-free

Size: 96 Well With Removable Strips

Human P4HB ELISA Kit PicoKine® (96 Tests). Quantitate Human P4HB in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

P4HB is a multifunctional and highly abundant enzyme that is part of the protein disulfide isomerase family. It is mapped to 17q25.3. When present as a tetramer consisting of two alpha and two beta subunits, P4HB has a role in hydroxylation of prolyl residues in preprocollagen. P4HB is a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>155</td><td>171</td><td>167</td><td>157</td><td>162</td><td>6.68</td><td>4.1%</td></tr><tr><td>Sample 2</td><td>785</td><td>728</td><td>688</td><td>729</td><td>732</td><td>34.52</td><td>4.7%</td></tr><tr><td>Sample 3</td><td>2454</td><td>2351</td><td>2283</td><td>2430</td><td>2379</td><td>67.50</td><td>2.8%</td></tr></table>*number of samples for each test n=16.

Synonyms

Protein disulfide-isomerase; PDI; Cellular thyroid hormone-binding protein; Prolyl 4-hydroxylase subunit beta; p55; P4HB; ERBA2L; PDI; PDIA1; PO4DB

Gene Name

prolyl 4-hydroxylase subunit beta

Gene ID

5034

UniProt

P07237

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: D18-L508

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA).

Detection Range

93.8 pg/ml - 6,000 pg/ml

Sensitivity

<15 pg/ml

Function

This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modificatio

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human P4HB

Anticoagulant

heparin or EDTA

Subcellular Location

Endoplasmic reticulum, Endoplasmic reticulum lumen, Cell membrane, Peripheral membrane protein, Melanosome

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