Rat CPE/Carboxypeptidase E ELISA Kit PicoKine®, Carrier-free

Rat CPE ELISA Kit PicoKine® (96 Tests). Quantitate Rat Cpe in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

CPE may play a role in post-translational processing of peptide hormones including preproopiomelanocortin. It is mapped to 16p13.This gene encodes a member of the M14 family of metallocarboxypeptidases. The encoded preproprotein is proteolytically processed to generate the mature peptidase. This peripheral membrane protein cleaves C-terminal amino acid residues and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin. This protein may also function independently of its peptidase activity, as a neurotrophic factor that promotes neuronal survival, and as a sorting receptor that binds to regulated secretory pathway proteins, including prohormones. Mutations in this gene are implicated in type 2 diabetes.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>72</td><td>65</td><td>65</td><td>65</td><td>66</td><td>3.03</td><td>4.5%</td></tr><tr><td>Sample 2</td><td>271</td><td>294</td><td>289</td><td>283</td><td>284</td><td>8.58</td><td>3.0%</td></tr><tr><td>Sample 3</td><td>858</td><td>870</td><td>828</td><td>820</td><td>844</td><td>20.63</td><td>2.4%</td></tr></table>*number of samples for each test n=16.

Synonyms

Carboxypeptidase E; CPE; Carboxypeptidase H; CPH; Enkephalin convertase; Prohormone-processing carboxypeptidase

Gene Name

carboxypeptidase E

Gene ID

25669

UniProt

P15087

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L43-F476

Tissue Specificity

Expressed in brain, heart and anterior pituitary gland. Also expressed in pancreatic islets and adrenal gland.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA).

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<50 pg/ml

Function

Removes residual C-terminal Arg or Lys remaining after initial endoprotease cleavage during prohormone processing.

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)