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Mouse CPE/Carboxypeptidase E ELISA Kit PicoKine®, Carrier-free

Selected Variant

EK1888-carrier-free

Size: 96 Well With Removable Strips

Mouse CPE ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Cpe in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

CPE encodes carboxypeptidase E, a prohormone-processing exopeptidase found in secretory granules of endocrine and neuroendocrine cells. It is mapped to 8 B3.1; 8 32.3 cM. The encoded preproprotein undergoes proteolytic processing to generate a mature, functional enzyme that cleaves the C-terminal basic residues of protein substrates. A missense mutation in this gene is responsible for the obesity phenotype in a mouse model known as the "fat mouse." Mice lacking the functional product of this gene exhibit impaired processing of multiple peptide hormones such as proinsulin, prodynorphin, proneurotensin, promelanin-concentrating hormone and pro-opiomelanocortin.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>91</td><td>94</td><td>82</td><td>89</td><td>89</td><td>4.41</td><td>4.9%</td></tr><tr><td>Sample 2</td><td>252</td><td>268</td><td>294</td><td>252</td><td>266</td><td>17.16</td><td>6.4%</td></tr><tr><td>Sample 3</td><td>873</td><td>901</td><td>1007</td><td>933</td><td>928</td><td>50.04</td><td>5.3%</td></tr></table>*number of samples for each test n=16.

Synonyms

Carboxypeptidase E; CPE; Carboxypeptidase H; CPH; Enkephalin convertase; Prohormone-processing carboxypeptidase

Gene Name

carboxypeptidase E

Gene ID

12876

UniProt

Q00493

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L43-F476

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA).

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<50 pg/ml

Function

Removes residual C-terminal Arg or Lys remaining after initial endoprotease cleavage during prohormone processing. Processes proinsulin.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant mouse CPE

Anticoagulant

heparin or EDTA

Subcellular Location

Secreted, secretory vesicle membrane, Peripheral membrane protein

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