Mouse SerpinE2 ELISA Kit PicoKine®, Carrier-free

Mouse SerpinE2 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Serpine2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) ,urine. Sensitivity: 0.1ng/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Glia-derived nexin is a protein that in humans is encoded by the SERPINE2 gene. This gene is mapped to 1 C4; 1 40.97 cM. SERPINE2 produced in Sf9 Baculovirus cells is a single,glycosylated polypeptide chain containing 386 aminoacids (20-397a.a.) andhaving a molecular mass of 42.9kDa (Molecular size on SDS-PAGE will appear atapproximately 40-57kDa).SERPINE2 is expressed with a 8 aminoacid His tag atC-Terminus and purified by proprietary chromatographic techniques. This gene displays neurite promoting activity and serine protease inhibitory activity.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>599</td><td>657</td><td>572</td><td>652</td><td>620</td><td>35.83</td><td>5.7%</td></tr><tr><td>Sample 2</td><td>2848</td><td>2654</td><td>2960</td><td>2710</td><td>2793</td><td>119.50</td><td>4.2%</td></tr><tr><td>Sample 3</td><td>10128</td><td>10127</td><td>9926</td><td>9025</td><td>9801</td><td>455.79</td><td>4.6%</td></tr></table>*number of samples for each test n=16.

Synonyms

Glia-derived nexin; GDN; Peptidase inhibitor 7; PI-7; Protease nexin 1; PN-1; Protease nexin I; Serine protease-inhibitor 4; Serpin E2; Serpine2; Pi7; Pn1; Spi4

Gene Name

serine (or cysteine) peptidase inhibitor, clade E, member 2

Gene ID

20720

UniProt

Q07235

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: S20-P397

Tissue Specificity

Most abundant in seminal vesicles.

Applications

ELISA

Standard

50 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA, citrate) ,urine.

Detection Range

0.78 ng/ml - 50 ng/ml

Sensitivity

<0.1 ng/ml

Function

Serine protease inhibitor with activity toward thrombin, trypsin, and urokinase. Promotes neurite extension by inhibiting thrombin. Binds heparin.

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)