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Mouse Plexin B2/PLXNB2 ELISA Kit PicoKine®, Carrier-free

Selected Variant

EK1882-carrier-free

Size: 96 Well With Removable Strips

Mouse Plexin B2 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Plxnb2 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 0.1ng/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Plexin-B2 is a protein that in humans is encoded by the PLXNB2 gene. It is mapped to 15 E3; 15 44.68 cM. Members of the B class of plexins, such as PLXNB2 are transmembrane receptors that participate in axon guidance and cell migration in response to semaphorins.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>29</td><td>30</td><td>31</td><td>28</td><td>29</td><td>1.11</td><td>3.8%</td></tr><tr><td>Sample 2</td><td>192</td><td>180</td><td>170</td><td>178</td><td>180</td><td>7.87</td><td>4.3%</td></tr><tr><td>Sample 3</td><td>447</td><td>475</td><td>514</td><td>455</td><td>472</td><td>25.90</td><td>5.4%</td></tr></table>*number of samples for each test n=16.

Synonyms

Plexin-B2; Plxnb2

Gene Name

plexin B2

Gene ID

140570

UniProt

B2RXS4

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: CHO; Immunogen sequence: L20-P1201

Tissue Specificity

Detected in macrophages from spleen and bone marrow (at protein level). Detected in granule cells in the developing cerebellum, dentate gyrus and olfactory bulb.

Applications

ELISA

Standard

50 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA).

Detection Range

0.78 ng/ml - 50 ng/ml

Sensitivity

<50 pg/ml

Function

Cell surface receptor for SEMA4C, SEMA4D and SEMA4G that plays an important role in cell-cell signaling. Binding to class 4 semaphorins promotes downstream activation of RHOA and phosphorylation of ERBB2 at 'Tyr-1248'. Required for normal differentiation

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant mouse Plexin B2

Anticoagulant

heparin or EDTA

Subcellular Location

Cell membrane, Single-pass type I membrane protein

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