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Human CPA2 ELISA Kit PicoKine®, Carrier-free

Selected Variant

EK1861-carrier-free

Size: 96 Well With Removable Strips

Human CPA2 ELISA Kit PicoKine® (96 Tests). Quantitate Human CPA2 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Carboxypeptidase A2 is an enzyme that in humans is encoded by the CPA2 gene. This gene is mapped to 7q32.2. Three different forms of human pancreatic procarboxypeptidase A have been isolated. The encoded protein represents the A2 form, which is a monomeric protein with different biochemical properties from the A1 and A3 forms. The A2 form of pancreatic procarboxypeptidase acts on aromatic C-terminal residues and is a secreted protein.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>108</td><td>117</td><td>106</td><td>116</td><td>111</td><td>4.81</td><td>4.3%</td></tr><tr><td>Sample 2</td><td>747</td><td>740</td><td>627</td><td>672</td><td>696</td><td>49.68</td><td>7.1%</td></tr><tr><td>Sample 3</td><td>2199</td><td>2420</td><td>2342</td><td>2125</td><td>2271</td><td>115.91</td><td>5.1%</td></tr></table>*number of samples for each test n=16.

Synonyms

Carboxypeptidase A2; CPA2;

Gene Name

carboxypeptidase A2

Gene ID

1358

UniProt

P48052

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: S115-Y419

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA).

Detection Range

93.6 pg/ml - 6,000 pg/ml

Sensitivity

<15 pg/ml

Function

Similar to that of carboxypeptidase A (EC 3.4.17.1), but with a preference for bulkier C-terminal residues.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human CPA2

Anticoagulant

heparin or EDTA

Subcellular Location

Secreted

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