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Human MANF ELISA Kit PicoKine®, Carrier-free

Selected Variant

EK1845-carrier-free

Size: 96 Well With Removable Strips

Human MANF ELISA Kit PicoKine® (96 Tests). Quantitate Human MANF in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 15 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

MANF is a 20kDa protein which belongs to the ARMET family. This gene is mapped to 3p21.2. MANF was originally known as an arginine-rich region protein which was extremely mutated in a large number of tumors. MANF Expression is induced during ER stress, signifying that MANF takes part in protein quality control during ER stress.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>156</td><td>148</td><td>167</td><td>169</td><td>160</td><td>8.51</td><td>5.3%</td></tr><tr><td>Sample 2</td><td>733</td><td>654</td><td>771</td><td>713</td><td>717</td><td>42.29</td><td>5.8%</td></tr><tr><td>Sample 3</td><td>2725</td><td>2678</td><td>2428</td><td>2740</td><td>2642</td><td>126.07</td><td>4.7%</td></tr></table>*number of samples for each test n=16.

Synonyms

Mesencephalic astrocyte-derived neurotrophic factor; Arginine-rich protein; Protein ARMET; MANF; ARMET; ARP

Gene Name

mesencephalic astrocyte derived neurotrophic factor

Gene ID

7873

UniProt

P55145

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L25-L182

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA).

Detection Range

93.6 pg/ml - 6,000 pg/ml

Sensitivity

<15 pg/ml

Function

Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents i

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human MANF

Anticoagulant

heparin or EDTA

Subcellular Location

Endoplasmic reticulum lumen, Secreted, Sarcoplasmic reticulum lumen

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