Human Plexin B3 ELISA Kit PicoKine®, Carrier-free

Human Plexin B3 ELISA Kit PicoKine® (96 Tests). Quantitate Human PLXNB3 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Plexin-B3 is a protein that in humans is encoded by the PLXNB3 gene. Ths gene is mapped to Xq28. The protein encoded by this gene is a member of the plexin family. It functions as a receptor for semaphorin 5A, and plays a role in axon guidance, invasive growth and cell migration. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>156</td><td>141</td><td>147</td><td>153</td><td>149</td><td>5.76</td><td>3.8%</td></tr><tr><td>Sample 2</td><td>984</td><td>848</td><td>932</td><td>931</td><td>923</td><td>48.70</td><td>5.2%</td></tr><tr><td>Sample 3</td><td>3201</td><td>3180</td><td>3318</td><td>2761</td><td>3115</td><td>211.03</td><td>6.7%</td></tr></table>*number of samples for each test n=16.

Synonyms

Plexin-B3; PLXNB3; KIAA1206; PLXN6

Gene Name

plexin B3

Gene ID

5365

UniProt

Q9ULL4

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: H45-Q1255

Tissue Specificity

Expression detected in Purkinje and granular cells in cerebellum, and in brain neocortex but not in corpus callosum. Expressed in glioma cells and embryonic kidney cells (at protein level). Expressed in brain, liver, pancreas and placenta, with weak expre

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA).

Detection Range

93.6 pg/ml - 6,000 pg/ml

Sensitivity

<15 pg/ml

Function

Receptor for SEMA5A that plays a role in axon guidance, invasive growth and cell migration. Stimulates neurite outgrowth and mediates Ca2+/Mg2+-dependent cell aggregation. In glioma cells, SEMA5A stimulation of PLXNB3 results in the disassembly of F-actin

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)