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Human Plexin B2/PLXNB2 ELISA Kit PicoKine®, Carrier-free

Selected Variant

EK1826-carrier-free

Size: 96 Well With Removable Strips

Human Plexin B2 ELISA Kit PicoKine® (96 Tests). Quantitate Human PLXNB2 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 0.1ng/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Members of the B class of plexins, such as PLXNB2 are transmembrane receptors that participate in axon guidance and cell migration in response to semaphorins.This gene is mapped to 22q13.33.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>1214</td><td>1018</td><td>1165</td><td>1017</td><td>1103</td><td>87.72</td><td>7.9%</td></tr><tr><td>Sample 2</td><td>4275</td><td>4570</td><td>4511</td><td>4741</td><td>4524</td><td>166.86</td><td>3.6%</td></tr><tr><td>Sample 3</td><td>19603</td><td>18628</td><td>19571</td><td>18283</td><td>19021</td><td>578.86</td><td>3.0%</td></tr></table>*number of samples for each test n=16.

Synonyms

Plexin-B2; MM1; PLXNB2; KIAA0315

Gene Name

plexin B2

Gene ID

23654

UniProt

O15031

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L20-L1838

Applications

ELISA

Standard

40 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA).

Detection Range

0.624 ng/ml - 40 ng/ml

Sensitivity

<0.1 ng/ml

Function

Cell surface receptor for SEMA4C, SEMA4D and SEMA4G that plays an important role in cell-cell signaling. Binding to class 4 semaphorins promotes downstream activation of RHOA and phosphorylation of ERBB2 at 'Tyr-1248'. Required for normal differentiation

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human Plexin B2

Anticoagulant

heparin or EDTA

Subcellular Location

Cell membrane, Single-pass type I membrane protein

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