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Mouse Plexin C1/PLXNC1 ELISA Kit PicoKine®, Carrier-free

Mouse Plexin C1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Plxnc1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate ). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

A Plexin is a protein which acts as a receptor for Semaphorin family signaling proteins. This gene is mapped to 10; 10 C2. It is classically known for its expression on the surface of axon growth cones and involvement in signal transduction to steer axon growth away from the source of Semaphorin. Plexin also has implications in development of other body systems by activating GTPase enzymes to induce a number of intracellular biochemical changes leading to a variety of downstream effects.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>168</td><td>149</td><td>160</td><td>163</td><td>160</td><td>6.96</td><td>4.3%</td></tr><tr><td>Sample 2</td><td>824</td><td>814</td><td>878</td><td>902</td><td>854</td><td>36.67</td><td>4.2%</td></tr><tr><td>Sample 3</td><td>2614</td><td>2932</td><td>2991</td><td>2861</td><td>2849</td><td>143.54</td><td>5.0%</td></tr></table>*number of samples for each test n=16.

Synonyms

Plexin-C1; Virus-encoded semaphorin protein receptor; CD232; Plxnc1; Vespr

Gene Name

plexin C1

Gene ID

54712

UniProt

Q9QZC2

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A35-T950

Tissue Specificity

Detected on dendritic cells, skin Langerhans cells and neutrophils.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA, citrate ).

Detection Range

93.8 pg/ml - 6,000 pg/ml

Sensitivity

<50 pg/ml

Function

Receptor for SEMA7A, for vaccinia virus semaphorin A39R and for herpesvirus Sema protein. Binding of semaphorins triggers cellular responses leading to the rearrangement of the cytoskeleton and to secretion of IL6 and IL8.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant mouse Plexin C1

Anticoagulant

heparin or EDTA

Subcellular Location

Membrane, Single-pass type I membrane protein

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