Human Ki67 ELISA Kit PicoKine®, Carrier-free

Human Ki67 ELISA Kit PicoKine® (96 Tests). Quantitate Human MKI67 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate ). Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Ki-67 (Proliferation-related Ki-67 antigen), also known as MKI67 or KIA, is a protein that in humans is encoded by the MKI67 gene. This gene is mapped to 10q26.2. From study of a panel of human-rodent somatic cell hybrids, it has been demonstrated that a gene involved in the expression of the MKI67 antigen is located on chromosome 10. By in situ hybridization, Fonatsch et al. (1991) regionalized the MKI67 gene to chromosome 10q25-qter. By FISH, Traut et al. Antigen KI-67 is a nuclear protein that is associated with and may be necessary for cellular proliferation. Furthermore it is associated with ribosomal RNA transcription. Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>15</td><td>17</td><td>17</td><td>15</td><td>16</td><td>1.00</td><td>6.2%</td></tr><tr><td>Sample 2</td><td>54</td><td>64</td><td>64</td><td>54</td><td>59</td><td>5.00</td><td>8.4%</td></tr><tr><td>Sample 3</td><td>222</td><td>213</td><td>228</td><td>245</td><td>227</td><td>11.68</td><td>5.1%</td></tr></table>*number of samples for each test n=16.

Synonyms

Proliferation marker protein Ki-67; Antigen identified by monoclonal antibody Ki-67; Antigen KI-67; Antigen Ki67; MKI67

Gene Name

marker of proliferation Ki-67

Gene ID

4288

UniProt

P46013

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: N3120-I3256

Tissue Specificity

Expression occurs preferentially during late G1, S, G2 and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected (at protein level). Present at highest level in G2 phase and during mitosis (at protein level). In interphase,

Applications

ELISA

Standard

1 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA, citrate ).

Detection Range

7.8 pg/ml - 500 pg/ml

Sensitivity

<1 pg/ml

Function

Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly. Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome su

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)