Human Endorepellin/HSPG2 ELISA Kit PicoKine®, Carrier-free

Human Endorepellin/HSPG2 ELISA Kit PicoKine® (96 Tests). Quantitate Human HSPG2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate ). Sensitivity: 0.05ng/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Perlecan (PLC) also known as HSPG2, is a protein that in humans is encoded by the HSPG2 gene. It is mapped to 1p36.12. Perlecan is highly conserved across species and the available data indicate that it has evolved from ancient ancestors by gene duplication and exonshuffling. Perlecan is a key component of the vascular extracellular matrix, here it interacts with a variety of other matrix components and helps to maintain the endothelial barrier function. It is a potent inhibitor of smooth muscle cell proliferation and is thus thought to help maintain vascular homeostasis. Perlecan can also promote growth factor (e.g., FGF2) activity and thus stimulate endothelial growth and re-generation.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>1098</td><td>1094</td><td>1079</td><td>1015</td><td>1071</td><td>33.38</td><td>3.1%</td></tr><tr><td>Sample 2</td><td>3228</td><td>3312</td><td>3233</td><td>3315</td><td>3272</td><td>41.55</td><td>1.2%</td></tr><tr><td>Sample 3</td><td>15773</td><td>17166</td><td>16982</td><td>16097</td><td>16504</td><td>584.53</td><td>3.5%</td></tr></table>*number of samples for each test n=16.

Synonyms

Basement membrane-specific heparan sulfate proteoglycan core protein; HSPG; Perlecan; PLC; HSPG2

Gene Name

heparan sulfate proteoglycan 2

Gene ID

3339

UniProt

P98160

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: E3687-S4391

Tissue Specificity

Found in the basement membranes.

Applications

ELISA

Standard

40 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA, citrate ).

Detection Range

0.624 ng/ml - 40 ng/ml

Sensitivity

<0.05 ng/ml

Function

Integral component of basement membranes. Component of the glomerular basement membrane (GBM), responsible for the fixed negative electrostatic membrane charge, and which provides a barrier which is both size- and charge-selective. It serves as an attachm

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)