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Human GSTA ELISA Kit PicoKine®, Carrier-free

Selected Variant

EK1625-carrier-free

Size: 96 Well With Removable Strips

Human GSTA ELISA Kit PicoKine® (96 Tests). Quantitate Human GSTA1 in cell culture supernatants,cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml . The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

GSTA encodes a member of a family of enzymes that function to add glutathione to target electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins, and products of oxidative stress. This action is an important step in detoxification of these compounds. This subfamily of enzymes has a particular role in protecting cells from reactive oxygen species and the products of peroxidation. Polymorphisms in this gene influence the ability of individuals to metabolize different drugs. This gene is located in a cluster of similar genes and pseudogenes on chromosome 6. Alternative splicing results in multiple transcript variants.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>298</td><td>279</td><td>301</td><td>294</td><td>293</td><td>8.45</td><td>2.8%</td></tr><tr><td>Sample . 2</td><td>1176</td><td>1300</td><td>1194</td><td>1293</td><td>1240</td><td>56.16</td><td>4.5%%</td></tr><tr><td>Sample 3</td><td>6136</td><td>5932</td><td>6351</td><td>5515</td><td>5983</td><td>308.40</td><td>5.1%</td></tr></table>*number of samples for each test n=16.

Synonyms

Glutathione S-transferase A2; GST HA subunit 2; GST class-alpha member 2; GST-gamma; GSTA2-2; GTH2; GSTA2; GST2

Gene Name

glutathione S-transferase alpha 1

Gene ID

2938

UniProt

P08263

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A2-F222

Tissue Specificity

Liver.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants,cell lysates, serum and plasma (heparin, EDTA).

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<10 pg/ml

Function

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human GSTA

Anticoagulant

heparin or EDTA

Subcellular Location

Cytoplasm.

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