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Human HRG ELISA Kit PicoKine®, Carrier-free

Selected Variant

EK1707-carrier-free

Size: 96 Well With Removable Strips

Human HRG ELISA Kit PicoKine® (96 Tests). Quantitate Human HRG in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml . The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

HRG contains two cystatin-like domains and is located in plasma and platelets. The physiological function has not been determined but it is known that the protein binds heme, dyes and divalent metal ions. The encoded protein also has a peptide that displays antimicrobial activity against C. albicans, E. coli, S. aureus, P. aeruginosa, and E. faecalis. It can inhibit rosette formation and interacts with heparin, thrombospondin and plasminogen. Two of the protein's effects, the inhibition of fibrinolysis and the reduction of inhibition of coagulation, indicate a potential prothrombotic effect. Mutations in this gene lead to thrombophilia due to abnormal histidine-rich glycoprotein levels.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>244</td><td>264</td><td>246</td><td>248</td><td>250</td><td>7.92</td><td>3.1%</td></tr><tr><td>Sample . 2</td><td>1957</td><td>2089</td><td>1985</td><td>1978</td><td>2002</td><td>51.13</td><td>2.5%%</td></tr><tr><td>Sample 3</td><td>4517</td><td>4462</td><td>4358</td><td>4566</td><td>4475</td><td>77.29</td><td>1.7%</td></tr></table>*number of samples for each test n=16.

Synonyms

Heme transporter HRG1; Heme-responsive gene 1 protein homolog; HRG-1; hHRG-1; Solute carrier family 48 member 1; SLC48A1; HRG1

Gene Name

histidine rich glycoprotein

Gene ID

3273

UniProt

Q6P1K1

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: V19-K525

Tissue Specificity

Highly expressed in the brain, kidney, heart and skeletal muscle. Moderately expressed in the liver, lung, placenta and small intestine.

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA).

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<10 pg/ml

Function

Heme transporter that regulates intracellular heme availability through the endosomal or lysosomal compartment.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human HRG

Anticoagulant

heparin or EDTA

Subcellular Location

Endosome membrane

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