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Human Serpin F2/alpha 2-Antiplasmin/SERPINF2 ELISA Kit PicoKine®, Carrier-free

Selected Variant

EK1712-carrier-free

Size: 96 Well With Removable Strips

Human Serpin F2/alpha 2-Antiplasmin/SERPINF2 ELISA Kit PicoKine® (96 Tests). Quantitate Human SERPINF2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml . The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Human SerpinF2 encodes a member of the serpin family of serine protease inhibitors. The protein is a major inhibitor of plasmin, which degrades fibrin and various other proteins. Consequently, the proper function of this gene has a major role in regulating the blood clotting pathway. Mutations in this gene result in alpha-2-plasmin inhibitor deficiency, which is characterized by severe hemorrhagic diathesis. Multiple transcript variants encoding different isoforms have been found for this gene.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>1883</td><td>2175</td><td>2014</td><td>2130</td><td>2050</td><td>113.14</td><td>5.5%</td></tr><tr><td>Sample . 2</td><td>10402</td><td>11339</td><td>11464</td><td>10592</td><td>10949</td><td>459.34</td><td>4.1%%</td></tr><tr><td>Sample 3</td><td>40754</td><td>39524</td><td>41038</td><td>45998</td><td>41828</td><td>2473.60</td><td>5.9%</td></tr></table>*number of samples for each test n=16.

Synonyms

Fibroblast growth factor-binding protein 1; FGF-BP; FGF-BP1; FGF-binding protein 1; FGFBP-1; Growth factor-binding protein 1; Fgfbp1

Gene Name

serpin family F member 2

Gene ID

5345

UniProt

P08697

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: N40-K491

Tissue Specificity

Expressed in gut, eye, thymus, skin, lung, tongue, Purkinje cells and cerebral chorioid plexus (at protein level).

Applications

ELISA

Standard

100 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Detection Range

1.56 ng/ml - 100 ng/ml

Sensitivity

<10 pg/ml

Function

Acts as a carrier protein that release fibroblast- binding factors (FGFs) from the extracellular matrix (EM) storage and thus enhance the mitogenic activity of FGFs. Enhances FGF2 signaling during tissue repair, angiogenesis and in tumor growth (By similarity). .

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human SerpinF2

Anticoagulant

heparin, EDTA or citrate

Subcellular Location

Secreted, extracellular space

Price

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