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Mouse Serpin A1 ELISA Kit PicoKine®, Carrier-free

Selected Variant

EK1711-carrier-free

Size: 96 Well With Removable Strips

Mouse Serpin A1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Serpina1c in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml . The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

SERPINA1 is also known as PI, A1A or AAT. This gene is mapped to 14q32.1. The protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>2876</td><td>2791</td><td>2438</td><td>2616</td><td>2680</td><td>168.37</td><td>6.2%</td></tr><tr><td>Sample . 2</td><td>13987</td><td>13721</td><td>13461</td><td>14393</td><td>13890</td><td>344.60</td><td>2.4%%</td></tr><tr><td>Sample 3</td><td>37231</td><td>37794</td><td>38247</td><td>41965</td><td>38809</td><td>1857.18</td><td>4.7%</td></tr></table>*number of samples for each test n=16.

Synonyms

Serpina1c; Dom3; Dom6; Spi1-3; Spi1-6; Alpha-1-antitrypsin 1-3; Alpha-1 protease inhibitor 3; Alpha-1 protease inhibitor 6; Alpha-1-antitrypsin 1-6; Serine protease inhibitor 1-3; Serine protease inhibitor 1-6; Serine protease inhibitor A1c; Serpin A1c

Gene Name

Alpha-1-antitrypsin 1-3

Gene ID

20702

UniProt

Q00896

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: E25-K413

Applications

ELISA

Standard

100 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA).

Detection Range

1.56 ng/ml - 100 ng/ml

Sensitivity

<10 pg/ml

Function

Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant mouse AAT

Anticoagulant

heparin or EDTA

Price

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