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Mouse GPC2 ELISA Kit PicoKine®, Carrier-free

Selected Variant

EK1715-carrier-free

Size: 96 Well With Removable Strips

Mouse GPC2 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Gpc2 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml . The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Glypican 2 (GPC2), also known cerebroglycan, is a protein which in humans is encoded by the GPC2 gene.Cerebroglycan is a glycophosphatidylinositol-linked integral membrane heparan sulfate proteoglycan found in the developing nervous system. Cerebroglycan participates in cell adhesion and is thought to regulate the growth and guidance of axons. Cerebroglycan has especially high affinity for laminin-1

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>128</td><td>121</td><td>126</td><td>111</td><td>121</td><td>6.57</td><td>5.4%</td></tr><tr><td>Sample . 2</td><td>499</td><td>417</td><td>473</td><td>425</td><td>453</td><td>33.89</td><td>7.4%%</td></tr><tr><td>Sample 3</td><td>1231</td><td>1236</td><td>1124</td><td>1165</td><td>1189</td><td>46.83</td><td>3.9%</td></tr></table>*number of samples for each test n=16.

Synonyms

Glypican-2; Secreted glypican-2; Gpc2

Gene Name

glypican 2 (cerebroglycan)

Gene ID

71951

UniProt

Q8BKV1

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: H22-S556

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA).

Detection Range

62.5 pg/ml - 4,000 pg/ml

Sensitivity

<10 pg/ml

Function

Cell surface proteoglycan that bears heparan sulfate. May fulfill a function related to the motile behaviors of developing neurons (By similarity). .

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant mouse SerpinF2

Anticoagulant

heparin or EDTA

Subcellular Location

Cell membrane ; Lipid-anchor, GPI-anchor ; Extracellular side .

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