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Rat FGFBP1 ELISA Kit PicoKine®, Carrier-free

Selected Variant

EK1695-carrier-free

Size: 96 Well With Removable Strips

Rat FGFBP1 ELISA Kit PicoKine® (96 Tests). Quantitate Rat Fgfbp1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml . The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Fibroblast growth factor-binding protein 1 is a protein that in humans is encoded by the FGFBP1 gene. This gene encodes a secreted fibroblast growth factor carrier protein. The encoded protein plays a critical role in cell proliferation, differentiation and migration by binding to fibroblast growth factors and potentiating their biological effects on target cells. The encoded protein may also play a role in tumor growth as an angiogenic switch molecule, and expression of this gene has been associated with several types of cancer including pancreatic and colorectal adenocarcinoma. A pseudogene of this gene is also located on the short arm of chromosome 5.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>112</td><td>108</td><td>117</td><td>112</td><td>112</td><td>3.19</td><td>2.8%</td></tr><tr><td>Sample . 2</td><td>566</td><td>629</td><td>674</td><td>611</td><td>620</td><td>38.71</td><td>6.2%%</td></tr><tr><td>Sample 3</td><td>2662</td><td>2454</td><td>2765</td><td>2801</td><td>2670</td><td>135.00</td><td>5.0%</td></tr></table>*number of samples for each test n=16.

Synonyms

Fibroblast growth factor-binding protein 1;FGF-BP;FGF-BP1;FGF-binding protein 1;Growth factor-binding protein 1;FGFBP-1;Fgfbp1

Gene Name

fibroblast growth factor binding protein 1

Gene ID

64535

UniProt

Q9QY10

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: E21-C238

Tissue Specificity

Expressed in gut, eye, thymus, skin, lung, tongue, Purkinje cells and cerebral chorioid plexus (at protein level).

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA).

Detection Range

78 pg/ml - 5,000 pg/ml

Sensitivity

<10 pg/ml

Function

Expressed in gut, eye, thymus, skin, lung, tongue, Purkinje cells and cerebral chorioid plexus (at protein level).

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant rat FGFBP1

Anticoagulant

heparin or EDTA

Price

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