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Human Pro-collagen I alpha 1/COL1A1 ELISA Kit PicoKine®, Carrier-free

Human Pro-collagen Iα1/COL1A1 ELISA Kit PicoKine® (96 Tests). Quantitate Human COL1A1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml . The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Collagen, type I, alpha 1, also known as COL1A1, is a human gene that encodes the major component of type I collagen, the fibrillar collagen found in most connective tissues, including cartilage. This gene is mapped to 17q21.33. And this gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>95</td><td>94</td><td>96</td><td>98</td><td>95</td><td>1.47</td><td>1.5%</td></tr><tr><td>Sample 2</td><td>317</td><td>320</td><td>299</td><td>298</td><td>308</td><td>10.06</td><td>3.2%</td></tr><tr><td>Sample 3</td><td>1043</td><td>1012</td><td>1051</td><td>1042</td><td>1037</td><td>14.84</td><td>1.4%</td></tr></table>*number of samples for each test n=16.

Synonyms

Collagen alpha-1 (I) chain; Alpha-1 type I collagen; COL1A1

Gene Name

collagen type I alpha 1 chain

Gene ID

P02452

UniProt

P02452

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: CHO; Immunogen sequence: Q23-K277 + G1094-L1464

Tissue Specificity

Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Detection Range

62.5 pg/ml - 4,000 pg/ml

Sensitivity

<10 pg/ml

Function

Type I collagen is a member of group I collagen (fibrillar forming collagen).

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human COL1A1

Anticoagulant

heparin, EDTA or citrate

Subcellular Location

Secreted, extracellular space, extracellular matrix

Frequently Asked Questions

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