Human TRKC ELISA Kit PicoKine®, Carrier-free

Human TRKC ELISA Kit PicoKine® (96 Tests). Quantitate Human NTRK3 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml . The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Tropomyosin receptor kinase C? (TrkC),?also known as?NT-3 growth factor receptor,?neurotrophic tyrosine kinase receptor type 3, or?TrkC tyrosine kinase?is a?protein?that in humans is encoded by the?NTRK3?gene.This gene encodes a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation and may play a role in the development of proprioceptive neurons that sense body position. Mutations in this gene have been associated with medulloblastomas, secretory breast carcinomas and other cancers. Several transcript variants encoding different isoforms have been found for this gene.?

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>330</td><td>342</td><td>297</td><td>342</td><td>327</td><td>18.41</td><td>5.6%</td></tr><tr><td>Sample 2</td><td>1741</td><td>1582</td><td>1616</td><td>1731</td><td>1667</td><td>69.63</td><td>4.1%</td></tr><tr><td>Sample 3</td><td>4941</td><td>4243</td><td>4806</td><td>4622</td><td>4653</td><td>262.39</td><td>5.6%</td></tr></table>*number of samples for each test n=16.

Synonyms

NT-3 growth factor receptor; GP145-TrkC; Trk-C; Neurotrophic tyrosine kinase receptor type 3; TrkC tyrosine kinase; NTRK3; TRKC

Gene Name

neurotrophic receptor tyrosine kinase 3

Gene ID

4916

UniProt

Q16288

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: C32-A428

Tissue Specificity

Widely expressed but mainly in nervous tissue. Isoform 2 is expressed at higher levels in adult brain than in fetal brain.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA).

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<10 pg/ml

Function

Receptor tyrosine kinase involved in nervous system and probably heart development. Upon binding of its ligand NTF3/neurotrophin-3, NTRK3 autophosphorylates and activates different signaling pathways, including the phosphatidylinositol 3-kinase/AKT and the MAPK pathways, that control cell survival and differentiation.

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)