Human Serpin A1/alpha 1-Antitrypsin ELISA Kit PicoKine®, Carrier-free

Human Serpin A1/alpha 1-Antitrypsin ELISA Kit PicoKine® (96 Tests). Quantitate Human SERPINA1 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10pg/ml . The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Alpha-1-antitrypsin?or?α1-antitrypsin? (A1AT,?A1A, or?AAT) is a?protein?belonging to the?serpin?superfamily. It is encoded in humans by the?SERPINA1?gene.?The?gene?is located on the long arm of the fourteenth?chromosome? (14q32.1).The protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene.Like all?serine protease inhibitors, A1AT has a characteristic?secondary structure?of?beta sheets?and?alpha helices.?Mutations?in these areas can lead to non-functional proteins that can?polymerise?and accumulate in the?liver (infantile hepatic cirrhosis).

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>2451</td><td>2297</td><td>2432</td><td>2102</td><td>2320</td><td>139.42</td><td>6%</td></tr><tr><td>Sample 2</td><td>15117</td><td>15111</td><td>14581</td><td>14540</td><td>14837</td><td>277.13</td><td>1.8%</td></tr><tr><td>Sample 3</td><td>38332</td><td>38692</td><td>42108</td><td>38880</td><td>39503</td><td>1516.83</td><td>3.8%</td></tr></table>*number of samples for each test n=16.

Synonyms

Alpha-1-antitrypsin; Alpha-1 protease inhibitor; Alpha-1-antiproteinase; Serpin A1; Short peptide from AAT; SPAAT; SERPINA1; AAT; PI; PRO0684; PRO2209

Gene Name

serpin family A member 1

Gene ID

5265

UniProt

P01009

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: E25-K418

Tissue Specificity

Ubiquitous. Expressed in leukocytes and plasma.

Applications

ELISA

Standard

100 ng/tube

Sample Type

cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Detection Range

1.56 ng/ml - 100 ng/ml

Sensitivity

<10 pg/ml

Function

Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)