Human Dermatopontin/DPT ELISA Kit PicoKine®, Carrier-free

Human Dermatopontin/DPT ELISA Kit PicoKine® (96 Tests). Quantitate Human DPT in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml . The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Dermatopontin?is a?protein?that in humans is encoded by the?DPT?gene. It’s an?extracellular matrix?protein with possible functions in cell-matrix interactions and matrix assembly. The protein is found in various tissues and many of its tyrosine residues are sulphated. Dermatopontin is postulated to modify the behavior of?TGF-beta?through interaction with?decorin.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>100</td><td>105</td><td>105</td><td>109</td><td>104</td><td>3.19</td><td>3%</td></tr><tr><td>Sample 2</td><td>977</td><td>1016</td><td>907</td><td>886</td><td>946</td><td>52.39</td><td>5.5%</td></tr><tr><td>Sample 3</td><td>2868</td><td>2711</td><td>2472</td><td>2675</td><td>2681</td><td>141.05</td><td>5.2%</td></tr></table>*number of samples for each test n=16.

Synonyms

Dermatopontin; Tyrosine-rich acidic matrix protein; TRAMP; DPT

Gene Name

dermatopontin

Gene ID

1805

UniProt

Q07507

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q19-V201

Tissue Specificity

Expressed in fibroblasts, heart, skeletal muscle, brain and pancreas. Expressed at an intermediate level in lung and kidney, and at a low level in liver and placenta. Expressed at a lower level in fibroblasts from hypertrophic scar lesional skin and in fibroblasts from patients with systemic sclerosis than in normal skin fibroblasts.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA).

Detection Range

93.7 pg/ml - 6,000 pg/ml

Sensitivity

<10 pg/ml

Function

Seems to mediate adhesion by cell surface integrin binding. May serve as a communication link between the dermal fibroblast cell surface and its extracellular matrix environment. Enhances TGFB1 activity. Inhibits cell proliferation. Accelerates collagen fibril formation, and stabilizes collagen fibrils against low-temperature dissociation (By similarity). .

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)