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Rat HE4/WFDC2 ELISA Kit PicoKine®, Carrier-free

Selected Variant

EK1624-carrier-free

Size: 96 Well With Removable Strips

Rat HE4/WFDC2 ELISA Kit PicoKine® (96 Tests). Quantitate Rat Wfdc2 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

WAP four-disulfide core domain protein 2, also known as Human Epididymis Protein 4 (HE4), is a protein that in humans is encoded by the WFDC2 gene. This gene encodes a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. This gene is expressed in pulmonary epithelial cells, and was also found to be expressed in some ovarian cancers. The encoded protein is a small secretory protein, which may be involved in sperm maturation.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>295</td><td>325</td><td>303</td><td>331</td><td>313</td><td>14.92</td><td>4.7%</td></tr><tr><td>Sample 2</td><td>1986</td><td>1810</td><td>2046</td><td>2132</td><td>1993</td><td>117.96</td><td>5.9%</td></tr><tr><td>Sample 3</td><td>4547</td><td>4620</td><td>4129</td><td>4297</td><td>4398</td><td>196.24</td><td>4.4%</td></tr></table>*number of samples for each test n=16.

Synonyms

WAP four-disulfide core domain protein 2; Epididymal secretory protein 4; RE4; Wfdc2

Gene Name

WAP four-disulfide core domain 2

Gene ID

286888

UniProt

Q8CHN3

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: E31-F168

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA).

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<10 pg/ml

Function

Broad range protease inhibitor.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant rat HE4

Anticoagulant

heparin or EDTA

Subcellular Location

Secreted.

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