Human AGT/Serpin A8/Angiotensinogen ELISA Kit PicoKine®, Carrier-free

Human AGT/Serpin A8/Angiotensinogen ELISA Kit PicoKine® (96 Tests). Quantitate Human AGT in cell culture supernatants, serum and plasma (EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

AGT (ANGIOTENSINOGEN), also called SERPINA8, is an α-2-globulin that is produced constitutively and released into the circulation mainly by the liver. ATG is a member of the serpin family, although it is not known to inhibit other enzymes, unlike most serpins. Angiotensinogen is also known as renin substrate. The AGT gene is mapped on 1q42.2. And the human angiotensinogen gene contains 5 exons. The expression of AGT and enzymes required for its conversion to angiotensin II in human adipose tissue. Mutations in this AGT gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene have also been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>364</td><td>349</td><td>378</td><td>353</td><td>361</td><td>11.24</td><td>3.1%</td></tr><tr><td>Sample 2</td><td>2379</td><td>2185</td><td>2390</td><td>2124</td><td>2269</td><td>117.06</td><td>5.1%</td></tr><tr><td>Sample 3</td><td>7370</td><td>7573</td><td>8346</td><td>7810</td><td>7774</td><td>364.72</td><td>4.6%</td></tr></table>*number of samples for each test n=16.

Synonyms

Angiotensinogen; Serpin A8; Angiotensin-1; Angiotensin 1-10; Angiotensin I; Ang I; Angiotensin-2; Angiotensin 1-8; Angiotensin II; Ang II; Angiotensin-3; Angiotensin 2-8; Angiotensin III; Ang III; Des-Asp[1]-angiotensin II; Angiotensin-4; Angiotensin 3-8; Angiotensin IV; Ang IV; Angiotensin 1-9; Angiotensin 1-7; Angiotensin 1-5; Angiotensin 1-4; AGT; SERPINA8

Gene Name

angiotensinogen

Gene ID

183

UniProt

P01019

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: D34-A485

Tissue Specificity

Expressed by the liver and secreted in plasma.

Applications

ELISA

Standard

20 ng/tube

Sample Type

cell culture supernatants, serum and plasma (EDTA).

Detection Range

312 pg/ml - 20,000 pg/ml

Sensitivity

<10 pg/ml

Function

Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis.

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)