Rat TFF3/ITF ELISA Kit PicoKine®, Carrier-free

Rat TFF3 ELISA Kit PicoKine® (96 Tests). Quantitate Rat Tff3 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Trefoil factor 3 is a protein that in humans is encoded by the TFF3 gene. It is mapped to 21q22.3. Members of the trefoil family can interact with mucins and have an influence on mucus viscosity. They also promote migration of epithelial cells, are linked to antiapoptosis, induce cell scattering, trigger chemotaxis, and participate in immune responses. This gene is a marker of columnar epithelium and is expressed in a variety of tissues including goblet cells of the intestines and colon. It has been found that TFF3 is the most highly upregulated gene in 19 poorly differentiated endometrioid endometrial carcinomas (G3-EECs) compared with normal endometrium biopsies.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>89</td><td>102</td><td>99</td><td>102</td><td>98</td><td>5.33</td><td>5.4%</td></tr><tr><td>Sample 2</td><td>213</td><td>209</td><td>247</td><td>247</td><td>229</td><td>18.05</td><td>7.8%</td></tr><tr><td>Sample 3</td><td>889</td><td>919</td><td>932</td><td>949</td><td>922</td><td>21.94</td><td>2.3%</td></tr></table>*number of samples for each test n=16.

Synonyms

Trefoil factor 3; Intestinal trefoil factor; rITF; Polypeptide P1.B; rP1.B; Tff3; Itf

Gene Name

trefoil factor 3

Gene ID

25563

UniProt

Q03191

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A23-F81

Tissue Specificity

Expressed in goblet cells of the intestines, and colon, in paraventricular hypothalamus and supraoptic nuclei. Weakly expressed in gastric epithelial cells (at protein level). Expressed by goblet cells of small and large intestinal epithelia, kidney and stomach. Expressed in the paraventricular hypothalamus, arcuate nucleus and amygdala of the brain. Weakly expressed in gastric epithelial cells.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA).

Detection Range

31.2 pg/ml - 2,000 pg/ml

Sensitivity

<10 pg/ml

Function

Involved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen) (By similarity).

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)