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Human IL-19/Interleukin-19 ELISA Kit PicoKine®, Carrier-free

Selected Variant

EK1638-carrier-free

Size: 96 Well With Removable Strips

Human IL-19 ELISA Kit PicoKine® (96 Tests). Quantitate Human IL19 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Interleukin 19 (IL19) is a protein that in humans is encoded by the IL19 gene. The protein encoded by this gene is a cytokine that belongs to the IL-10 cytokine subfamily. This cytokine is found to be preferentially expressed in monocytes. It can bind the interleukin-20 receptor complex and lead to the activation of the signal transducer and activator of transcription 3 (STAT3). A similar cytokine in mouse is reported to up-regulate the expression of IL6 and TNF-alpha and induce apoptosis, which suggests a role of this cytokine in inflammatory responses. Alternatively spliced transcript variants encoding the distinct isoforms have been described.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>57</td><td>58</td><td>51</td><td>55</td><td>55</td><td>2.68</td><td>4.8%</td></tr><tr><td>Sample 2</td><td>290</td><td>309</td><td>293</td><td>295</td><td>296</td><td>7.29</td><td>2.4%</td></tr><tr><td>Sample 3</td><td>1018</td><td>1068</td><td>897</td><td>1026</td><td>1002</td><td>63.66</td><td>6.3%</td></tr></table>*number of samples for each test n=16.

Synonyms

Interleukin-19; IL-19; Melanoma differentiation-associated protein-like protein; NG.1; IL19; ZMDA1

Gene Name

interleukin 19

Gene ID

29949

UniProt

Q9UHD0

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: L25-A177

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Detection Range

31.2 pg/ml - 2,000 pg/ml

Sensitivity

<10 pg/ml

Function

May play some important roles in inflammatory responses. Up-regulates IL-6 and TNF-alpha and induces apoptosis (By similarity).

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human IL-19

Anticoagulant

heparin or EDTA

Subcellular Location

Secreted.

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