Human PLA2G7/Lp-PLA2 ELISA Kit PicoKine®, Carrier-free

Human PLA2G7/Lp-PLA2 ELISA Kit PicoKine® (96 Tests). Quantitate Human PLA2G7 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Lipoprotein-associated phospholipase A2 (Lp-PLA2) also known as platelet-activating factor acetylhydrolase (PAF-AH) is a phospholipase A2 enzyme that in humans is encoded by the PLA2G7 gene. Lp-PLA2 is a 45-kDa protein of 441 amino acids. It is one of several PAF acetylhydrolases. The protein encoded by this gene is a secreted enzyme that catalyzes the degradation of platelet-activating factor to biologically inactive products. Defects in this gene are a cause of platelet-activating factor acetylhydrolase deficiency. Two transcript variants encoding the same protein have been found for this gene.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>1179</td><td>1334</td><td>1212</td><td>1128</td><td>1213</td><td>75.86</td><td>6.2%</td></tr><tr><td>Sample 2</td><td>7607</td><td>7415</td><td>7511</td><td>7063</td><td>7399</td><td>205.52</td><td>2.7%</td></tr><tr><td>Sample 3</td><td>22955</td><td>22970</td><td>21188</td><td>24226</td><td>22834</td><td>1081.67</td><td>4.7%</td></tr></table>*number of samples for each test n=16.

Synonyms

Platelet-activating factor acetylhydrolase; PAF acetylhydrolase; 1-alkyl-2-acetylglycerophosphocholine esterase; 2-acetyl-1-alkylglycerophosphocholine esterase; Group-VIIA phospholipase A2; gVIIA-PLA2; LDL-associated phospholipase A2; LDL-PLA (2); PAF 2-acylhydrolase; PLA2G7; PAFAH

Gene Name

phospholipase A2 group VII

Gene ID

7941

UniProt

Q13093

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: F22-N441

Tissue Specificity

Plasma.

Applications

ELISA

Standard

50 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA).

Detection Range

0.78 ng/ml - 50 ng/ml

Sensitivity

<20 pg/ml

Function

Modulates the action of platelet-activating factor (PAF) by hydrolyzing the sn-2 ester bond to yield the biologically inactive lyso-PAF. Has a specificity for substrates with a short residue at the sn-2 position. It is inactive against long-chain phospholipids.

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)