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Mouse Mimecan ELISA Kit PicoKine®, Carrier-free

Selected Variant

EK1617-carrier-free

Size: 96 Well With Removable Strips

Mouse Mimecan ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Ogn in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 12pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Osteoglycin (also called mimecan) is a protein that in humans is encoded by the OGN gene. This gene encodes a protein which induces ectopic bone formation in conjunction with transforming growth factor beta. This protein is a small proteoglycan which contains tandem leucine-rich repeats (LRR). The level of expression of this gene has been correlated with enlarged hearts and more specifically left ventricular hypertrophy. Mimecan belongs to a family of small leucine-rich proteoglycans (SLRPs). The mimecan gene was expressed at a moderate level in the mouse pituitary gland by Northern blot analysis. Expression of mimecan mRNA and protein is also observed in the human anterior pituitary gland. The OGN gene was mapped to chromosome 9q22.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>190</td><td>198</td><td>185</td><td>187</td><td>190</td><td>4.94</td><td>2.6%</td></tr><tr><td>Sample 2</td><td>372</td><td>421</td><td>383</td><td>410</td><td>396</td><td>19.78</td><td>4.9%</td></tr><tr><td>Sample 3</td><td>1477</td><td>1456</td><td>1506</td><td>1663</td><td>1525</td><td>81.34</td><td>5.3%</td></tr></table>*number of samples for each test n=16.

Synonyms

Mimecan; Osteoglycin; Ogn; Og;

Gene Name

osteoglycin

Gene ID

18295

UniProt

Q62000

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A20-F298

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA).

Detection Range

62.5 pg/ml - 4,000 pg/ml

Sensitivity

<12 pg/ml

Function

Induces bone formation in conjunction with TGF-beta-1 or TGF-beta-2. .

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant mouse Mimecan

Anticoagulant

heparin or EDTA

Subcellular Location

Secreted, extracellular space, extracellular matrix .

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