Human PD-L2/B7-DC ELISA Kit PicoKine®, Carrier-free

Human PD-L2/B7-DC ELISA Kit PicoKine® (96 Tests). Quantitate Human PDCD1LG2 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 12pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Programmed cell death 1 ligand 2 (also known as PD-L2, B7-DC) is a protein that in humans is encoded by the PDCD1LG2 gene. It is mapped to chromosome 9p24.2. PD-L2 is one of two ligands for programmed death-1 (PD-1), a member of the CD28 family of immuno-receptors. Northern blot analysis detect wide expression of PDL2, with highest levels in placenta, heart, pancreas, lung, and liver, and lower levels in spleen, lymph nodes, and thymus. PDL2 negatively regulates T cells and plays an essential role in immune tolerance.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>133</td><td>126</td><td>127</td><td>117</td><td>125</td><td>5.71</td><td>4.5%</td></tr><tr><td>Sample 2</td><td>396</td><td>453</td><td>452</td><td>415</td><td>429</td><td>24.44</td><td>5.6%</td></tr><tr><td>Sample 3</td><td>998</td><td>945</td><td>1041</td><td>1035</td><td>1004</td><td>38.22</td><td>3.8%</td></tr></table>*number of samples for each test n=16.

Synonyms

Programmed cell death 1 ligand 2; PD-1 ligand 2; PD-L2; PDCD1 ligand 2; Programmed death ligand 2; Butyrophilin B7-DC; B7-DC; CD273; PDCD1LG2; B7DC, CD273, PDCD1L2, PDL2;

Gene Name

programmed cell death 1 ligand 2

Gene ID

80380

UniProt

Q9BQ51

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L20-T220

Tissue Specificity

Highly expressed in heart, placenta, pancreas, lung and liver and weakly expressed in spleen, lymph nodes and thymus.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA).

Detection Range

62.5 pg/ml - 4,000 pg/ml

Sensitivity

<12 pg/ml

Function

Involved in the costimulatory signal, essential for T- cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production (By similarity). .

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)