Mouse PON1/Paraoxonase 1 ELISA Kit PicoKine®, Carrier-free

Mouse PON1/Paraoxonase 1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Pon1 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Serum paraoxonase/arylesterase 1 (PON1) also known as aromatic esterase 1 or serum aryldialkylphosphatase 1 is an enzyme that in humans is encoded by the PON1 gene. The PON gene was mapped to chromosome 7q21-q22, and the mouse Pon1 gene was mapped to the proximal end of chromosome 6. PON1 is responsible for hydrolysing organophosphate pesticides and nerve gasses. Polymorphisms in the PON1 gene significantly affect the catalytic ability of the enzyme. PON1 (paraoxonase 1) is also a major anti-atherosclerotic component of high-density lipoprotein (HDL).

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>364</td><td>310</td><td>330</td><td>311</td><td>328</td><td>21.85</td><td>6.6%</td></tr><tr><td>Sample 2</td><td>1139</td><td>1282</td><td>1324</td><td>1250</td><td>1248</td><td>68.58</td><td>5.4%</td></tr><tr><td>Sample 3</td><td>5447</td><td>5579</td><td>4941</td><td>5080</td><td>5261</td><td>260.23</td><td>4.9%</td></tr></table>*number of samples for each test n=16.

Synonyms

Serum paraoxonase/arylesterase 1; PON 1; 3.1.1.2

Gene Name

paraoxonase 1

Gene ID

18979

UniProt

P52430

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A30-L355

Tissue Specificity

Plasma, liver, kidney, heart, brain, small intestine and lung. In the plasma, associated with HDL.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin).

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<10 pg/ml

Function

Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification.

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)