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Human MMP-10/Stromelysin-2 ELISA Kit PicoKine®, Carrier-free

Selected Variant

EK0467-carrier-free

Size: 96 Well With Removable Strips

Human MMP10 ELISA Kit PicoKine® (96 Tests). Quantitate Human MMP10 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Stromelysin-2 also known as matrix metalloproteinase-10 (MMP-10) or transin-2 is an enzyme that in humans is encoded by the MMP10 gene. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades proteoglycans and fibronectin. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>150</td><td>142</td><td>133</td><td>133</td><td>139</td><td>7.08</td><td>5%</td></tr><tr><td>Sample 2</td><td>657</td><td>650</td><td>733</td><td>643</td><td>670</td><td>36.27</td><td>5.4%</td></tr><tr><td>Sample 3</td><td>2257</td><td>2665</td><td>2447</td><td>2386</td><td>2438</td><td>147.54</td><td>6%</td></tr></table>*number of samples for each test n=16.

Synonyms

Stromelysin-2; SL-2; 3.4.24.22; Matrix metalloproteinase-10; MMP-10; Transin-2; MMP10; STMY2;

Gene Name

matrix metallopeptidase 10

Gene ID

4319

UniProt

P09238

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Y18-C476

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin).

Detection Range

78 pg/ml - 5,000 pg/ml

Sensitivity

<10 pg/ml

Function

Can degrade fibronectin, gelatins of type I, III, IV, and V; weakly collagens III, IV, and V. Activates procollagenase.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human MMP-10

Anticoagulant

heparin

Subcellular Location

Secreted, extracellular space, extracellular matrix .

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