Human HGFA ELISA Kit PicoKine®, Carrier-free

Human HGFA ELISA Kit PicoKine® (96 Tests). Quantitate Human HGFAC in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Hepatocyte growth factor activator, also known as HGFA, is a protein that in humans is encoded by the HGFAC gene. This gene encodes a member of the peptidase S1 protein family. The encoded protein is first synthesized as an inactive single-chain precursor before being activated to a heterodimeric form by endoproteolytic processing. It acts as serine protease that converts hepatocyte growth factor to the active form. Alternative splicing results in multiple transcript variants.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>62</td><td>62</td><td>64</td><td>63</td><td>62</td><td>0.82</td><td>1.3%</td></tr><tr><td>Sample 2</td><td>304</td><td>296</td><td>265</td><td>269</td><td>283</td><td>16.8</td><td>5.9%</td></tr><tr><td>Sample 3</td><td>830</td><td>851</td><td>870</td><td>915</td><td>866</td><td>31.37</td><td>3.6%</td></tr></table>*number of samples for each test n=16.

Synonyms

Hepatocyte growth factor activator;HGF activator;HGFA;3.4.21.-;Hepatocyte growth factor activator short chain;Hepatocyte growth factor activator long chain;HGFAC;

Gene Name

Hepatocyte growth factor activator

Gene ID

3083

UniProt

Q04756

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q36-S655

Tissue Specificity

Liver.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

31.2 pg/ml - 2,000 pg/ml

Sensitivity

<10 pg/ml

Function

Activates hepatocyte growth factor (HGF) by converting it from a single chain to a heterodimeric form.

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)