Human Sclerostin/SOST ELISA Kit PicoKine®, Carrier-free

Human Sclerostin/SOST ELISA Kit PicoKine® (96 Tests). Quantitate Human SOST in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Sclerostin is a protein that in humans is encoded by the SOST gene. It is a secreted glycoprotein with a C-terminal cysteine knot-like (CTCK) domain and sequence similarity to the DAN (differential screening-selected gene aberrative in neuroblastoma) family of bone morphogenetic protein (BMP) antagonists. Additionally, Sclerostin is produced by the osteocyte and has anti-anabolic effects on bone formation. The SOST gene maps to chromosome 17q12-q21.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>89</td><td>84</td><td>85</td><td>97</td><td>88</td><td>5.11</td><td>5.8%</td></tr><tr><td>Sample 2</td><td>215</td><td>183</td><td>209</td><td>209</td><td>204</td><td>12.36</td><td>6%</td></tr><tr><td>Sample 3</td><td>883</td><td>875</td><td>1058</td><td>953</td><td>942</td><td>73.39</td><td>7.7%</td></tr></table>*number of samples for each test n=16.

Synonyms

Sclerostin;SOST;UNQ2976/PRO7455/PRO7476;

Gene Name

Sclerostin

Gene ID

50964

UniProt

Q9BQB4

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q24-Y213

Tissue Specificity

Widely expressed at low levels with highest levels in bone, cartilage, kidney, liver, bone marrow and primary osteoblasts differentiated for 21 days. Detected in the subendothelial layer of the aortic intima (at protein level). .

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

31.2 pg/ml - 2,000 pg/ml

Sensitivity

<10 pg/ml

Function

Negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation. .

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)