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Mouse KLK1/Kallikrein 1 ELISA Kit PicoKine®, Carrier-free

Mouse KLK1 / Kallikrein 1 / Tissue kallikrein ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Klk1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 12pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Kallikrein-1, also known as tissue kallikrein, is a protein that in humans is encoded by the KLK1 gene. This serine protease generates Lys-bradykinin by specific proteolysis of kininogen-1. KLK1 is a member of the peptidase S1 family. Its gene is mapped to 19q13.3. In all, it has got 262-amino acids which contain a putative signal peptide, followed by a short activating peptide and the protease domain. The protein is mainly found in kidney, pancreas, and salivary gland, showing a unique pattern of tissue-specific expression relative to other members of the family. KLK1 is implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>150</td><td>154</td><td>170</td><td>150</td><td>156</td><td>8.24</td><td>5.2%</td></tr><tr><td>Sample 2</td><td>602</td><td>649</td><td>645</td><td>637</td><td>633</td><td>18.55</td><td>2.9%</td></tr><tr><td>Sample 3</td><td>1476</td><td>1672</td><td>1722</td><td>1744</td><td>1653</td><td>105.74</td><td>6.3%</td></tr></table>*number of samples for each test n=16.

Synonyms

Kallikrein-1;3.4.21.35;Glandular kallikrein K1;KAL-B;Renal kallikrein;Tissue kallikrein-6;mGK-6;Klk1;Klk-6, Klk6;

Gene Name

Kallikrein-1

Gene ID

16612

UniProt

P15947

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: I25-D261

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

62.5 pg/ml - 4,000 pg/ml

Sensitivity

<12 pg/ml

Function

Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

28775 MW

Specificity

Natural and recombinant mouse KLK1

Anticoagulant

heparin or EDTA

Protein Name

Kallikrein-1

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