Human Siglec5/Cd170 ELISA Kit PicoKine®, Carrier-free

Human Siglec5/Cd170 ELISA Kit PicoKine® (96 Tests). Quantitate Human SIGLEC5 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Sialic acid-binding Ig-like lectin 5 is a protein that in humans is encoded by the SIGLEC5 gene. This gene encodes a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. These cell surface lectins are characterized by structural motifs in the immunoglobulin (Ig)-like domains and sialic acid recognition sites in the first Ig V set domain. The encoded protein is a member of the CD33-related subset of Siglecs and inhibits the activation of several cell types including monocytes, macrophages and neutrophils. Binding of group B Streptococcus (GBS) to the encoded protein plays a role in GBS immune evasion.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>93</td><td>103</td><td>103</td><td>92</td><td>97</td><td>5.26</td><td>5.4%</td></tr><tr><td>Sample 2</td><td>282</td><td>301</td><td>334</td><td>293</td><td>302</td><td>19.39</td><td>6.4%</td></tr><tr><td>Sample 3</td><td>1180</td><td>1269</td><td>1248</td><td>1310</td><td>1251</td><td>47.04</td><td>3.7%</td></tr></table>*number of samples for each test n=16.

Synonyms

Sialic acid-binding Ig-like lectin 5;Siglec-5;CD33 antigen-like 2;Obesity-binding protein 2;OB-BP2;OB-binding protein 2;CD170;SIGLEC5;CD33L2, OBBP2;

Gene Name

Sialic acid-binding Ig-like lectin 5

Gene ID

8778

UniProt

O15389

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: E17-L441

Tissue Specificity

Expressed by monocytic/myeloid lineage cells. Found at high levels in peripheral blood leukocytes, spleen, bone marrow and at lower levels in lymph node, lung, appendix, placenta, pancreas and thymus. Expressed by monocytes and neutrophils but absent from leukemic cell lines representing early stages of myelomonocytic differentiation.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

46.9 pg/ml - 3,000 pg/ml

Sensitivity

<15 pg/ml

Function

Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds equally to alpha-2,3-linked and alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)