Human Klotho beta/KLB ELISA Kit PicoKine®, Carrier-free

Human Klotho beta ELISA Kit PicoKine® (96 Tests). Quantitate Human KLB in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Klotho beta, a divergent structural member of the glycosidase I superfamily, is expressed primarily in the liver and pancreas, with lower expression in adipose tissue. It is mapped to chromosome 4. The Klotho beta mediated interaction of human FGF19 (mouse FGF15) with FGF Receptor 4 in the liver negatively regulates bile acid synthesis by controlling the secretion of two key bile acid synthase genes, cholesterol 7-alpha hydroxylase (Cyp7a1) and sterol 12-alpha hydroxylase (Cyp8b1). Klotho beta is also an essential cofactor for FGF21 activity and the Klotho gene family may have evolved to confer tissue-specific bioactivity on FGF19 subfamily members.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>277</td><td>256</td><td>255</td><td>290</td><td>269</td><td>14.73</td><td>5.4%</td></tr><tr><td>Sample . 2</td><td>1292</td><td>1377</td><td>1293</td><td>1260</td><td>1305</td><td>43.36</td><td>3.3%%</td></tr><tr><td>Sample 3</td><td>4214</td><td>3960</td><td>4302</td><td>3984</td><td>4115</td><td>146.59</td><td>3.5%</td></tr></table>*number of samples for each test n=16.

Synonyms

Beta-klotho;BKL;BetaKlotho;Klotho beta-like protein;KLB;

Gene Name

Beta-klotho

Gene ID

152831

UniProt

Q86Z14

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: F53-L997

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<50 pg/ml

Function

Contributes to the transcriptional repression of cholesterol 7-alpha-hydroxylase (CYP7A1), the rate-limiting enzyme in bile acid synthesis. Probably inactive as a glycosidase. Increases the ability of FGFR1 and FGFR4 to bind FGF21 (By similarity). .

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)