Human Afamin ELISA Kit PicoKine®, Carrier-free

Human Afamin ELISA Kit PicoKine® (96 Tests). Quantitate Human AFM in cell culture supernatants, serum and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Afamin is a protein that in humans is encoded by the AFM gene. This gene is a member of the albumin gene family, which is comprised of four genes that localize to chromosome 4 in a tandem arrangement. These four genes encode structurally-related serum transport proteins that are known to be evolutionarily related. The protein encoded by this gene is regulated developmentally, expressed in the liver and secreted into the bloodstream.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>221</td><td>236</td><td>257</td><td>237</td><td>237</td><td>12.79</td><td>5.3%</td></tr><tr><td>Sample 2</td><td>658</td><td>655</td><td>603</td><td>647</td><td>640</td><td>22.16</td><td>3.4%</td></tr><tr><td>Sample 3</td><td>3779</td><td>3418</td><td>3836</td><td>3710</td><td>3685</td><td>160.89</td><td>4.3%</td></tr></table>*number of samples for each test n=16.

Synonyms

Afamin;Alpha-albumin;Alpha-Alb;AFM;ALB2, ALBA;

Gene Name

Afamin

Gene ID

173

UniProt

P43652

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L22-N599

Tissue Specificity

High level detected in plasma but also in extravascular fluids such as follicular and cerebrospinal fluids (at protein level). .

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and urine.

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<10 pg/ml

Function

Vitamin E binding protein. May transport vitamin E in body fluids under conditions where the lipoprotein system is not sufficient. May be involved in the regulation and transport of vitamin E at the blood-brain barrier. .

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)