Human Serpin A7 ELISA Kit PicoKine®, Carrier-free

Human Serpin A7 ELISA Kit PicoKine® (96 Tests). Quantitate Human SERPINA7 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Thyroxine-binding globulin (TBG), also known as SERPINA7, is the major thyroid hormone transport protein in serum. There are three proteins including thyroxine-binding globulin (TBG), transthyretin and albumin responsible for carrying the thyroid hormones thyroxine (T4) and 3,5,3'-triiodothyronine (T3) in the bloodstream. This gene belongs to the serpin family in genomics, but the protein has no inhibitory function like many other members of the serpin family. Mutations in this gene result in TGB deficiency, which has been classified as partial deficiency, complete deficiency, and excess, based on the level of serum TBG. Alternatively spliced transcript variants encoding different isoforms have been found, but the full-length nature of these variants has not been determined. Using DNA blot hybridization, it is detected that there is a single-copy TBG gene located on the long arm of the X chromosome.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>81</td><td>87</td><td>98</td><td>94</td><td>90</td><td>6.51</td><td>7.2%</td></tr><tr><td>Sample 2</td><td>314</td><td>310</td><td>300</td><td>278</td><td>300</td><td>13.95</td><td>4.6%</td></tr><tr><td>Sample 3</td><td>875</td><td>1044</td><td>945</td><td>949</td><td>953</td><td>60.09</td><td>6.3%</td></tr></table>*number of samples for each test n=16.

Synonyms

Thyroxine-binding globulin;Serpin A7;T4-binding globulin;SERPINA7;TBG;

Gene Name

Thyroxine-binding globulin

Gene ID

6906

UniProt

P05543

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A21-A415

Tissue Specificity

Expressed by the liver and secreted in plasma.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<10 pg/ml

Function

Major thyroid hormone transport protein in serum.

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)