Rat beta IG-H3/TGFBI ELISA Kit PicoKine®, Carrier-free

Rat beta IG-H3/TGFBI ELISA Kit PicoKine® (96 Tests). Quantitate Rat TGFBI in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Transforming growth factor, beta-induced, 68kDa, also known as TGFBI (initially called BIGH3, BIG-H3), is a protein which in humans is encoded by the TGFBI gene. It is mapped to 5q31.1. This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. It is induced by transforming growth factor-beta and acts to inhibit cell adhesion.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>308</td><td>274</td><td>298</td><td>308</td><td>297</td><td>13.89</td><td>4.6%</td></tr><tr><td>Sample 2</td><td>1355</td><td>1497</td><td>1581</td><td>1353</td><td>1446</td><td>97.15</td><td>6.7%</td></tr><tr><td>Sample 3</td><td>5623</td><td>6215</td><td>5716</td><td>5905</td><td>5864</td><td>226.3</td><td>3.8%</td></tr></table>*number of samples for each test n=16.

Synonyms

Transforming growth factor-beta-induced protein ig-h3;Beta ig-h3;Kerato-epithelin;RGD-containing collagen-associated protein;RGD-CAP;TGFBI;BIGH3;

Gene Name

Transforming growth factor-beta-induced protein ig-h3

Gene ID

116487

UniProt

Q15582

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: G24-G685

Tissue Specificity

Highly expressed in the corneal epithelium. .

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<15 pg/ml

Function

Binds to type I, II, and IV collagens. This adhesion protein may play an important role in cell-collagen interactions. In cartilage, may be involved in endochondral bone formation.

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)