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Human AGP1/alpha 1 acid glycoprotein ELISA Kit PicoKine®, Carrier-free

Selected Variant

EK1486-carrier-free

Size: 96 Well With Removable Strips

Human AGP1/alpha 1 acid glycoprotein ELISA Kit PicoKine® (96 Tests). Quantitate Human ORM1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 150pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Alpha-1-acid glycoprotein, or orosomucoid, is a relatively abundant plasma protein synthesized in the liver. It has a molecular weith of 40,000 Da and is a single polypeptide chain of about 180 amino acids. AGP is considered an acute phase reactant; its plasma levels increase several fold during acute infections. It belongs to the lipocalin family and binds numerous basic and neutral lipophilic drugs and steroid hormones. And this gene is located in 9q34.3-qter.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>2696</td><td>2424</td><td>2599</td><td>2810</td><td>2632</td><td>141.53</td><td>5.3%</td></tr><tr><td>Sample 2</td><td>12095</td><td>11209</td><td>12283</td><td>11042</td><td>11657</td><td>539.13</td><td>4.6%</td></tr><tr><td>Sample 3</td><td>38792</td><td>45455</td><td>38596</td><td>41095</td><td>40984</td><td>2761.77</td><td>6.7%</td></tr></table>*number of samples for each test n=16.

Synonyms

Alpha-1-acid glycoprotein 1;AGP 1;Orosomucoid-1;OMD 1;ORM1;AGP1;

Gene Name

Alpha-1-acid glycoprotein 1

Gene ID

5004

UniProt

P02763

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q19-S201

Tissue Specificity

Expressed by the liver and secreted in plasma.

Applications

ELISA

Standard

100 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

1.56 ng/ml - 100 ng/ml

Sensitivity

<150 pg/ml

Function

Functions as transport protein in the blood stream. Binds various ligands in the interior of its beta-barrel domain. Also binds synthetic drugs and influences their distribution and availability in the body. Appears to function in modulating the activity of the immune system during the acute-phase reaction. .

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

23512 MW

Specificity

Natural and recombinant human AGP1

Anticoagulant

heparin or EDTA

Subcellular Location

Secreted.

Protein Name

Alpha-1-acid glycoprotein 1

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