Mouse HAI-2/SPINT2 ELISA Kit PicoKine®, Carrier-free

Mouse HAI-2/SPINT2 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Spint2 in cell culture supernatants, serum, plasma (heparin, EDTA) and cell lysates. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Kunitz-type protease inhibitor 2 is an enzyme inhibitor that in humans is encoded by the SPINT2 gene. This gene encodes a transmembrane protein with two extracellular Kunitz domains that inhibits a variety of serine proteases. The protein inhibits HGF activator which prevents the formation of active hepatocyte growth factor. And this gene is a putative tumor suppressor, and mutations in this gene result in congenital sodium diarrhea. Multiple transcript variants encoding different isoforms have been found for this gene.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>61</td><td>61</td><td>62</td><td>57</td><td>60</td><td>1.92</td><td>3.2%</td></tr><tr><td>Sample 2</td><td>240</td><td>243</td><td>264</td><td>249</td><td>249</td><td>9.24</td><td>3.7%</td></tr><tr><td>Sample 3</td><td>837</td><td>921</td><td>842</td><td>963</td><td>890</td><td>53.38</td><td>5.9%</td></tr></table>*number of samples for each test n=16.

Synonyms

Kunitz-type protease inhibitor 2;Hepatocyte growth factor activator inhibitor type 2;HAI-2;Spint2;Hai2;

Gene Name

Kunitz-type protease inhibitor 2

Gene ID

20733

UniProt

Q9WU03

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: R30-K140

Tissue Specificity

Isoform 2 is more predominantly expressed than isoform 1.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum, plasma (heparin, EDTA) and cell lysates.

Detection Range

31.2 pg/ml - 2,000 pg/ml

Sensitivity

<10 pg/ml

Function

Inhibitor of HGF activator.

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)